RELATED READING: Chapter 3, Pages 69-73 OBJECTIVES Upon completion of this exercise, appropriate discussion, and related readings, the student will be able to: Depending upon the type of test to be performed, there are many ways to collect blood, urine, and other body fluids. Usually a specific type of collection vessel is used to produce a specimen that is suitable for a particular type of analysis. For example, iodoacetate is placed in phlebotomy tubes to
prevent glycolysis. To collect unclotted blood a chemical such as oxalate, EDTA, or heparin must be added to prevent coagulation. Other types of phlebotomy tubes, such as those used for trace metal analysis, are designed to reduce possible contamination of the sample from exogenous metals. GLOSSARY Red top -a term indicating a phlebotomy tube containing no anticoagulant or preservative used to collect serum. Term refers to the color of the rubber stopper on the tube. Also- “green top”, “purple top”, etc. MATERIALS
PROCEDURE
OPTIONAL EXERCISE: HEMOLYSISMATERIALS
PROCEDURE
RESULTS PART A:
RESULTS PART B
*Use equation on following page. Calculation of approximate hemoglobin (Hb) concentrations in the hemolysates: Use basic dilution equation as follows: \[(conc_{i} \; Hb) (vol \ldotp \; dil \ldotp) = (conc_{f} \; Hb) (final\; dil \ldotp \; vol \ldotp)\] where conci Hb = concentration of Hb in blood (150 g/L) where concf Hb = concentration of Hb in diluted solutions (#1-#5) vol. dil. = volume of blood that is diluted final dil. vol. = final volume that blood is diluted out to (mL) This equation can be rearranged to read: \[conc_{f} \; Hb = \frac{(conc_{i}\; Hb)(vol \ldotp \; dil \ldotp)}{(final\; dil \ldotp \; vol \ldotp)}\] DISCUSSION QUESTIONS
What color tube and stopper is used to collect specimens for serum analysis?Serum is usually collected in mottled red/gray, gold, or cherry red-top tubes, and red-top tubes are occasionally used.
What color phlebotomy tube is used to collect specimens for serum quizlet?Serum or Plasma: red top and lavender top. Draw in prewarmed tube and clot at 37 degrees C.
Which tubes should be used to collect blood for serum testing quizlet?What color tubes are used to collect serum or clotted blood? 21-22g is most frequently used by phlebotomists. 16-18g is used for collecting donor blood.
Which tests require the use of a collection tube with a green stopper?Light green-top tube (lithium heparin)
This tube contains lithium heparin and gel separator used for the collection of heparinized plasma for routine chemistry tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.
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